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Assay Method Information

Assay Name:  Binding Assay
Description:  Stable and optimal assay conditions were determined by cross-titrating GST-AKAP18δ and biotinylated PLB using 10 μg/ml glutathione acceptor beads and 10 μg/ml streptavidin donor beads in an AlphaScreen assay. The excitation wavelength was 680 nm, with the emission wavelength being 520-560 nm. Signal intensity in each well was registered and the optimal concentration to use was chosen to be the concentration prior to the peak of the signal.
Affinity data for this assay
 

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Last update November 1, 2007
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