Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The production of NADPH from NADP+ and benzyl alcohol and xylitol was monitored by an increase in NADPH fluorescence (ex: 340 nm; em: 460 nm) using an SLM-Aminco 4800C fluorometer. For each inhibitor, at least three different inhibitor concentrations that bracketed Ki from 0.5- to 4-fold were used to determine Ki. For any one inhibitor concentration, the initial velocities were measured in duplicated or triplicate for at least five different substrate concentrations bracketing the apparent Km. Ki was determined by initial velocities to a competitive form of Michaelis-Menton equation.
Affinity data for this assay
 

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