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Assay Method Information

Assay Name:  Cell Adhesion Assay for Specificity of Inhibitors
Description:  The ligand was immobilized on 96-well flat microtiter in PBS buffer solution overnight, and the wells were blocked with 1% BSA in HBSS buffer solution without Ca2+ containing Mg2+ for 1 h. Cells in the same buffer solution without BSA were labeled with incubation of CMFDA for 30 min. After centrifugation and washing, cells were resuspended in the same buffer solution and incubating in the presence of different concentrations of inhibitors for 15 min. Cells were then added to the wells and continuously incubated for additional 30 min. Unbound cells were washed out, and bound cells were lysed by the addition of 0.5% Triton X-100. The plates were read using a Cytofluor 2350 fluorescence plate reader (Millipore, Bedford, MA) with 485 nm (excitation) and 530 nm (emission).
Affinity data for this assay
 

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Last update November 1, 2007
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