Assay Method Information

Assay Name:  MAO Inhibition Assay
Description:  A continuous spectrophotometric assay that monitors the rate of oxidation of the nonselective MAO substrate kynuramine into 4-hydroxyquinoline was used. clorgyline or selegiline was preincubated with the suspension for 5 min to measure MAO-B or MAO-A activity, respectively. The formation of 4-hydroxyquinoline was followed at 314 nm using a Kontron UVIKON 941 spectrophotometer. IC50 values were determined and calculated from a hyperbolic equation. Each assay was performed in triplicate on four to six separate homogenates.
Affinity data for this assay
 

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