BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Fluorescence Polarization Assay and FL5.12 Cellular Assay
Description:  The binding affinities (Ki) of compounds were determined using fluorescence polarization assays (FPA) that measure their ability to competitively displace a Bad-derived peptide from Bcl-xL. Compound efficacy (EC50) in a cellular context was evaluated by testing their ability to reverse the protection from cytokine withdrawal afforded by overexpression of Bcl-xL in the IL-3 dependent murine pro-B cell line FL5.12.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.