Assay Method Information | |
| AMACR Inhibition Assay |
Description: | AMACR activity was determined by monitoring the interconversion of the (25R/S)-isomer of THC-CoA. Reaction product was analyzed by resolution of (25S)-or (25R)-THC-CoA using HPLC, which was carried out with a reversed-phase C18-column and a linear gradient of methanol in potassium phosphate buffer (50 mM, pH 5.3). Kinetic parameters were determined using direct linear plot analysis. |
Affinity data for this assay | |
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