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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  Inhibition activity was determined using an assay that followed the production of PR-ATP spectrophotometrically. Enzymatic reaction was initiated by adding the substrate PRPP to reaction mixtures containing HisG enzyme and test compounds, and absorbance of product was monitored at 290 nm for 5 min until it formed a plateau.
Affinity data for this assay
 

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Last update November 1, 2007
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