Assay Method Information | |
| Enzyme Inhibition Assay |
Description: | Inhibition activity was determined using an assay that followed the production of PR-ATP spectrophotometrically. Enzymatic reaction was initiated by adding the substrate PRPP to reaction mixtures containing HisG enzyme and test compounds, and absorbance of product was monitored at 290 nm for 5 min until it formed a plateau. |
Affinity data for this assay | |
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