| Assay Method Information | |
| | In Vitro BACE1 Activity Assay |
| Description: | The assay was performed using a 96-well format on a HPLC equipped with four 96-well plate holders. After the reaction was terminated, the solution was filtered, and the filtrate was subjected to HPLC analysis. The substrate and product were monitored by fluorescence detection (340nm excitation, 440 nm emission).In the assessment of BACE-1 inhibitors, the percentage of remaining enzyme activity in titrations was calculated based on the area of the product peak compared with the sum of the areas of product and substrate peaks. All titration curves were fitted with a four-parameter logistic equation to determine the IC50 values of the compounds, and all results were derived from at least three independent measurements. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |