Assay Method Information | |
| ROCK Kinase Inhibition Assay |
Description: | The assay of Rock-1 activity involved incubation with peptide substrate and ATP33, and the incorporation of P33 into the peptide was quantified by Scintillation Proximity Assay (SPA - Amersham Pharmacia). For IC50 determination, compounds were assayed over an eleven point dilution range with a concentration in the assay of 50 uM to 0.8 nM. IC50 values were calculated by bespoke curve fitting software. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |