BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Homogeneous Time-Resolved Fluorescence (HTRF) Enzyme Assay
Description:  The assay uses purified baculovirus-expressed GST-VEGFR2 interacting with biotinylated peptide substrates. HTRF is based on the proximity of europium cryptate (donor fluorophore)-labeled antiphosphotyrosine and streptavidin labeled with APC (allophycocyanin), which have been brought together by a binding reaction. When the two entities come into close proximity and upon excitation, energy transfer occurs and APC re-emits at 665 nm. The IC50 values for inhibitors were determined by a four-parameter sigmoidal curve fit.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.