| Assay Method Information | |
| | APE1 Inhibition Assay |
| Description: | To determine the extent of abasic residue cleavage by APE1, recombinant APE1 was preincubated with the potential inhibitors in reaction buffer. Then, the 5-end 32P-labeled oligonucleotide substrate was added. The conversion of incision product was quantitated using PhosphorImager / ImageQuant 5.2 system. The IC50 values were determined by plotting the logarithm of drug concentration against percent inhibition of enzymatic activity. The optimum concentration of APE1 protein in the assay is the concentration at which there is complete conversion of the abasic-site containing oligonucleotide substrate to its cleaved product without subsequent exonuclease activity. |
| Affinity data for this assay | |
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