| Assay Method Information | |
| | PARP-1 Enzyme Inhibition Assay |
| Description: | To assess the inhibitory activity of novel inhibitors, the PARP-1 enzyme assay was carried out in reaction mixture consisting of Escherichia coli strain B typeVIII DNA, [adenylate-32P]-NAD, PARP-1, and various concentrations of PARP inhibitors. The reaction mixture was incubated at room temperature for 10 min, and the reaction was stopped with saturated ammonium sulfate, and filtered over MAIP filter plates. The incorporated radioactivity was determined by liquid scintillation counting. |
| Affinity data for this assay | |
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