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Assay Method Information

Assay Name:  Protease Inhibition Assay
Description:  Sensitivity of HIV-1 protease activity to protease inhibitors was determined by a peptide substrate cleavage assay. Substrates and cleavage fragments were separated by reverse-phase HPLC, detected by absorbance at 215 nm, and quantified by comparison with a synthetic product standard. For highly potent inhibitors, their Ki values were analyzed by a mathematical model for tight-binding inhibitors, in which the concentration of inhibitor is less than or approximately equal to the enzyme concentration.
Affinity data for this assay
 

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Last update November 1, 2007
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