Assay Method Information

Assay Name:  Screening for Inhibitors of the Mevalonate Pathway in Streptococcus Pneumoniae - DPM-DC Dose Response
Description:  Southern Research Molecular Libraries Screening Center (SRMLSC) Southern Research Institute (Birmingham, Alabama) NIH Molecular Libraries Screening Centers Network (MLSCN) Assay Provider: Dr. Thomas S. Leyh, Albert Einstein College of Medicine of Yeshiva University Streptococcus pneumoniae takes the lives of nearly 4,000 people daily and antibiotic resistant strains are becoming an increasing problem. Because of this, the discovery of drugs targeting novel pathways has become increasingly important. The mevalonate pathway produces isopentenyl diphosphate (the molecular building block of isoprenoids) and is essential for the survival of the pathogen in mouse lung and serum. The biosynthesis of isopentenyl diphosphate involves three consecutive reactions that are catalyzed by the enzymes mevalonate kinase (MK; E.C. 2.7.1.36), phosphomevalonate kinase (PMK; E.C. 2.7.4.2), and diphosphomevalonate decarboxylase (DPM-DC; E.C. 4.1.1.33). DPM-DC catalyzes the ATP-dependent conversion of (R
Affinity data for this assay
 

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