| Assay Method Information | |
| | PARP-1 ELISA Assay |
| Description: | The kit is based on ELISA. 10 μl sample/well, 15 μl PARP enzyme/well and PARP cocktail 25 μl/well with a total volume of 50 μl/well were added into a 96-well plate coated with histone. Meanwhile, blank control (without enzyme and sample) and negative control (without sample) were set. The plate was incubated at room temperature for 60 min; washed with 1×PBS (+0.1% TritonX-100) for twice; Strep-HRP 50 μl/well was added and incubated at room temperature for 60 min; and then washed with 1×PBS (+0.1% TritonX-100) for twice, chemiluminescence substrate 100 μl/well was added for measurement. The inhibition rate was calculated according to the value of chemiluminiscence intensity RLU (relative light unit), inhibition rate=[1−(RLU sample−RLU blank)/(RLU negative−RLU blank)]×100%. Each sample was gradiently diluted into eight concentrations, each concentration was set in a single well, IC50 values were calculated using 4 Parameter Logistic Model in XLfit software according to the inhibition rate of samples. |
| Affinity data for this assay | |
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