| Assay Method Information | |
| | High Throughput Screening Assay for Hsc70 Inhibitors |
| Description: | Sanford-Burnham Center for Chemical Genomics (SBCCG) Sanford-Burnham Medical Research Institute (San Diego, CA) NIH Molecular Libraries Screening Centers Network (MLSCN) Over-expression of molecular chaperones occurs commonly in cancers and provides protection from a wide variety of cellular stresses, both endogenous and iatrogenic. Molecular chaperones also play important roles in maintaining the activity of several signal-transducing proteins and transcriptions factors involved in malignant transformation. The human genome contains nine Hsp70-family genes. These chaperones include Hsp70 and Hsc70, which are commonly over-expressed in cancers and which confer resistance to myriad cellular stresses, including cytotoxic chemotherapy. This work's aim is to identify chemical probes of Hsc70 through a fluorescence polarization assay (FPA) using Fluorescein-labeled ATP. Hsc70 screening was performed at the Sanford-Burnham Center for Chemical Genomics (SBCCG) as part of the Molecular |
| Affinity data for this assay | |
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