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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  Inhibition of E. coli KARI (ketol-acid reductoisomerase) is time dependent, and activity was measured by the decrease in A340 at 30 C in solutions containing 0.2 mM NADH, 1 mM MgCl2, substrate 2-acetolactate and inhibitor in 0.1 M phosphate buffer at pH 8.0.
Affinity data for this assay
 

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Last update November 1, 2007
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