| Assay Method Information | |
| | Inhibition Assay |
| Description: | All compounds were screened for inhibitory activity against Lactobacillus casei thymidylate synthase (LcTS) and Escherichia coli thymidylate synthase (EsTS). The inhibition experiments were conducted by measuring the effects of the inhibitor at different concentrations on the initial velocity of the enzyme in the presence of a limited amount of the folate substrate. |
| Affinity data for this assay | |
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