Assay Method Information

Assay Name:  ChEMBL_1499557 (CHEMBL3584415)
Description:  Inhibition of human recombinant PAK1 kinase domain using coumarin/fluorescein-labeled FRET peptide as substrate assessed as substrate phosphorylation at Ser/Thr19 preincubated for 10 mins followed by ATP addition measured after 60 mins by Z'-LYTE assay
Affinity data for this assay
 

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