Assay Method Information | |
| ChEMBL_1657492 (CHEMBL4006962) |
Description: | Inhibition of recombinant human N-terminal truncated GST-tagged DHFR (31 to 395 residues) expressed in Escherichia coli BL21(DE3) pyrD using DHO as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by DCIP oxidation based CoQ10 enzyme coupled assay |
Affinity data for this assay | |
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