| Assay Method Information | |
| | Inhibition Assay |
| Description: | Nucleotide hydrolysis was monitored by mixing enzyme and substrate with a rapid kinetic accessory (Hi-Tech Scientific) attached to a spectrophotometer (Cary 50). Protons, released through the hydrolysis of nucleotides, were neutralized with similar pKa and monitored spectrophotometrically at the absorbance peak of the basic form of the indicator. |
| Affinity data for this assay | |
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