Assay Method Information | |
| Archibald assay |
Description: | Briefly, 0.5-50 mM L-arginine (pH 8.5) was added to a solution of 50 mM 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS) (pH 8.5) and 100 μM MnCl2. The reaction (200 μL) was initiated by adding 1 μM SmARG at 21°C and terminated after 1 min using 30 μL of a 3:1 (v/v) concentrated acid/dye solution. |
Affinity data for this assay | |
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