Assay Method Information

Assay Name:  Quantitative Reverse Transcriptase Assay
Description:  We used quantitative reverse-transcriptase PCR to measure the RNA levels of certain AMP genes (lysozyme, gallerimycin, moricin and cecropin) in caterpillars of M. sexta and the greater waxmoth G. mellonella challenged with Serratia entomophila or Salmonella enterica, respectively, following injection of different urea lipid compounds.
Affinity data for this assay
 

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