BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Radioligand Binding Assays
Description:  For the A2a receptor binding study, the incubation contained 10 mg of the original tissue weight of the striatal membranes, 4 nM [3H]NECA, 50 nM CPA, 10 mM MgCl2, 0.2 units/mL adenosine deaminase, the test compound and 1% DMSO. For the A1 receptor binding study, the incubations contained 5 mg of the original tissue weight of the whole brain membranes, 0.1 nM [3H]DPCPX, 0.2 units/mL adenosine deaminase, the test compound and 1% DMSO. The incubations were vortexed and incubated for 60 min at 25 C in a shaking waterbath. Half an hour after incubation was started, the incubations were vortexed again. The incubations were terminated via filtration. The tubes were washed twice with 4 mL ice-cold Tris buffer and the filters were washed once more with 4mL ice-cold Tris buffer. The damp filters were place in scintillation vials and 4 mL of scintillation fluid was added. The vials were shaken and incubated for two hours before being counted.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.