| Assay Method Information | |
| | ADP-GloTM Assay |
| Description: | The protein kinase assays used to determine IC50 value were performed using ADP-GloTM assay kit. The assay was started by incubating the reaction mixture in a 96-well plate at 30 °C for 30 min. After the 30 min incubation period, the assay was terminated by the addition of 25 mL of ADPGloTM Reagent. The 96-well plate was shaken and then incubated for 40 min at ambient temperature, 50 mL of kinase detection reagent was added, the 96-well reaction plate was then read using ADP-GloTM luminescences protocol on GloMaxTM plate reader. Blank control waqs set up that included all the assay components except the addition of appropriate substrate (replace with equal volume of kinase assay buffer). |
| Affinity data for this assay | |
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