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Assay Method Information

Assay Name:  Circular Dichroism (CD) Based Assays
Description:  MR activity was assayed using a CD-based assay by following the change in ellipticity of mandelate at 262 nm using a thermostated quartz cuvette with a 1-cm light path (unless otherwise indicated) as described by Sharp et al. Allassays (total volume of 2 mL) were conducted at 25 C in Na+ HEPES buffer (0.1 M, pH 7.5) containing MgCl2 (3.3 mM), BSA [0.005% (w/v)], and (R)-mandelate as the substrate. The concentration of MR was determined from its absorbance at 280 nm using an extinction coefficient of 53400 M^−1 cm^−1.
Affinity data for this assay
 

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Last update November 1, 2007
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