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Assay Method Information

Assay Name:  IC50 Assay
Description:  The reactions were done in a volume of 2 mL and constantly stirred using a magnetic stir bar at room temperature (23 C). Reactions with the crude, ammonium sulfate precipitated 5-LOX were carried out in 25 mM HEPES (pH 7.3), 0.3 mM CaCl2, 0.1 mM EDTA, 0.2 mM ATP, 0.01% Triton-X-100, and 10 lM AA. The concentrations of AA for 5-LOX assays were quantitatively determined by allowing the enzymatic reaction to go to completion.
Affinity data for this assay
 

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Last update November 1, 2007
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