| Assay Method Information | |
| | Direct Competition Assay |
| Description: | The Ki for each inhibitor was determined by direct competition assays under steady-state conditions. The initial velocity was measured in the presence of a constant concentration of enzyme (3 nM for VIM-2 and 4 nM for VIM-24) with increasing concentrations of inhibitor (5−60 μM) against a fixed concentration (5KM) of the indicator substrate, NCF, as previously described. The reactionswere started by addition of VIM-2 or VIM-24 to substrate or to mixtures of substrate and inhibitor. |
| Affinity data for this assay | |
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