Assay Method Information | |
| MAO Inhibition Assay |
Description: | IC50 values for the inhibition of MAO-A and MAO-B were determined using the recombinant human enzymes as enzyme sources, and kynuramine as non-selective MAO substrate. The concentrations of the MAO-generated 4-hydroxyquinoline were measured by fluorescence spectrophotometry (λex = 310; λem = 400 nm). The protocol for these experiments has been reported in detail in recent publications |
Affinity data for this assay | |
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