| Assay Method Information | |
| | MMP-2 Inhibition Assay |
| Description: | This esterase assay used 4-nitrophenylacetate as a substrate. Initially, the rhMMP-2 was diluted to 100 µg/mL with 1 mM APMA in assay buffer. Later, the reaction was incubated at 37℃ for 1 hour. Then the rhMMP-2 was diluted to 0.2 µg/mL in assay buffer, whereas the substrate was diluted to 20 µM by assay buffer. To each well of 96-well plate, 50 µL diluted rhMMP-2 was loaded along with 100 µL diverse concentration of test compounds. The reaction was started by adding 50 μL of 20 μM substrate to each well. After progress in kinetic mode for 5 minutes, the results were read at excitation and emission wavelengths of 320 nm and 405 nm, respectively. |
| Affinity data for this assay | |
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