| Assay Method Information | |
| | HDAC activity assays |
| Description: | Enzymatic characterization (Km determinations, Supplementary Fig. 3C) of zebrafish HDAC6 proteins (CD1-CD2; CD1H193A-CD2; CD1CD2H574A; CD1H193A- CD2H574A) was done with 50 nM of purified protein and increasing amounts of Fluor de Lys substrate, using an HDAC assay kit from Enzo biochemicals and following the manufacturer's instructions. Human HDAC6 protein (HsHDAC6 FL) was obtained from Reaction Biology Corporation. The fluorescence intensity was detected with a Spectromax Gemini plate reader (Molecular Devices). Curve fitting was done using GraphPad (GraphPad Software). The values expressed are the average of duplicate independent trials ± s.d. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |