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Assay Method Information

Assay Name:  Isothermal Titration Calorimetry (ITC)
Description:  Isothermal titration calorimetry experiments were performed using an iTC200 (GEHealthcare) at 25 C, with 15-20 μM ClpC1 (in 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 1 mM Tris(2-carboxyethyl) phosphine, 9% DMSO) in the sample cell. Typically, 19 injections of 2 μl of 150-200 μM CymA1 solution in the same bufferwere injected into the sample cell at 2.5-min intervals.
Affinity data for this assay
 

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Last update November 1, 2007
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