| Assay Method Information | |
| | IYD Binding Assay |
| Description: | Dissociation constants (Kd) for the ligands of IYD were determined by their ability to quench the fluorescence of the oxidized FMN (FMNox) (λex 450 nm, λem 523 nm) in the active site of IYD as previously described [Hu et al., J. Biol. Chem., 290:590-600; McTamney et al., J. Am. Chem. Soc., 131:14212-14213]. |
| Affinity data for this assay | |
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