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Assay Method Information

Assay Name:  8-[Fluo]-cAMP Fluorescence Polarization Binding Assay
Description:  The ability of the R subunit basic patch mutants to bind cAMP in the absence of the C subunit was tested using the fluorescent cAMP analogue, 8-[fluo]-cAMP (Biolog), used at a final concentration of 10 nM. Wells were titrated with a rangeof R subunit concentrations (from 0 to 125 nM), which were diluted with FP assay buffer.
Affinity data for this assay
 

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Last update November 1, 2007
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