| Assay Method Information | |
| | 8-[Fluo]-cAMP Fluorescence Polarization Binding Assay |
| Description: | The ability of the R subunit basic patch mutants to bind cAMP in the absence of the C subunit was tested using the fluorescent cAMP analogue, 8-[fluo]-cAMP (Biolog), used at a final concentration of 10 nM. Wells were titrated with a rangeof R subunit concentrations (from 0 to 125 nM), which were diluted with FP assay buffer. |
| Affinity data for this assay | |
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