| Assay Method Information | |
| | SPR Screening Assay |
| Description: | The rat NTSR1 variant NTSR1-H4 was first expressed in E. coli using a derivative of the vector pRG/III-hsMBP. This construct had a C-terminally fused Avi-tag, which, due to its cytoplasmic location, is biotinylated in vivo in E. coli [Egloff et al., Proc. Natl. Acad. Sci. U.S.A., 111:E655-E662]. The incorporation of biotin allowed a direct coupling of the purified receptor on a streptavidin-coated surface. All surface plasmon resonance (SPR) measurements were performed on a Biacore T100 instrument. Screening was performed against immobilized free receptor and blocked receptor (bound to NTS8-13), as well as a blank reference surface. Kd obtained from kinetic analysis. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |