Assay Method Information

Assay Name:  Fluorescence Polarization Assay
Description:  Fluorescence anisotropy was measured on a Tecan infinite M1000 plate reader with an excitation at 470 nm and emission at 525 nm, using Greiner 96 flat bottom black plate. Fixed probe concentration (0.1 μM) is added to an increasing concentration of galectin (0.001 to 200 μM). Measurements were undertaken at RT or at 4 °C after 1 hour incubation at rt under 100 rpm.
Affinity data for this assay
 

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