| Assay Method Information | |
| | Resonance Raman Assay |
| Description: | Samples for solution resonance Raman spectroscopic studies were prepared withfinal concentrations of 75 μM WT DHP B and 3.75 mM azole in 100 mM KPi (pH 7 or 9) containing 10% MeOH (v/v) and then transferred to a 5 mm outside diameter glass NMR tube. Anaerobic samples were prepared inside a glovebox in the same fashion using septum-capped NMR tubes. Spectra were recorded by Soret band excitation (400−430 nm, 60 mW power) as previously described. [Barrios et al., J. Am. Chem. Soc., 136:7914-7925] |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |