| Assay Method Information | |
| | Surface Plasmon Resonance (SPR) Assay |
| Description: | Surface plasmon resonance (SPR) assays were carried out on a BIAcore 3000 instrument (Biacore, Inc). Briefly, purified hTNFα was dissolved at 320 μg/ml in PBS, pH 7.0, and coupled to a carboxyl surface (HR-5 chip from HRBio, Inc.) by following the manufacturer's instructions. For the equilibrium-binding experiments, the flow rate was set to 10 μl min^-1, and 50μl of the compound sample was injected. For the kinetic experiments, the flow rate was also 10 μl min^-1, and 50 μl was injected for kinetic assay. |
| Affinity data for this assay | |
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