| Assay Method Information | |
| | Isothermal Titration Calorimetry (ITC) |
| Description: | An aqueous solution of the fragment to be tested (1 mM) was prepared containing NaCl (300 mM), Tris.HCl (20 mM, pH = 8.0), d6-DMSO (10% v/v) and glycerol (to match the 10% v/v glycerol content in the EthR stock solution). A separate aqueous solution of EthR (50 μM) containing NaCl (300 mM), Tris.HCl (20 mM, pH = 8.0) and DMSO-d6 (10% v/v) was prepared and placed in the sample cell of a MicroCal iTC200 microcalorimeter (GE Healthcare). The fragment solution was then titrated to the EthR solution over 19 or 39 injections (first injection of 0.4 μl and subsequent injections of 2.0 μL or 1.0 μL). |
| Affinity data for this assay | |
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