| Assay Method Information | |
| | AChE Inhibition Bioassay |
| Description: | AChE enzymatic activity was measured using an adaptation of the method previously described [Ingkaninan et al., J. Ethnopharmacol., 89:261-264]; 98 μl of 50 mM Tris-HCl buffer (pH 8), 30 μl of a solution sample of the inhibitor, at different concentrations in methanol, and 7.5 μl of AChE solution containing 0.26 U/ml were mixed in a microplate and left to incubate for 15 min. Subsequently, 22.5 μl of 0.023 mg/ml AChI and 142 μl of 3 mM DTNB were added. The initial rate of the enzymatic reaction was followed by reading the absorbance at 405 nm during the first 5 min of reaction. Samples were prepared in a range of concentrations of the compounds in water (choline caffeate, choline trolox, choline cinnamate) or in an aqueous solution of 50% methanol (choline 3,4-dimethoxicinnamate, choline rosmarinate). A control reaction was carried out using the sample solvent instead of sample and it was considered 100% activity. |
| Affinity data for this assay | |
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