Assay Method Information

Assay Name:  LOX Activity Assay
Description:  Enzyme activity was monitored by UV analysis. 0.05 cm^3 of enzyme solution was added to a cuvette containing 2 cm^3 linoleic acid solution and the appropriate amounts of buffer and inhibitor solutions in thermostatic water bath at 25°C. There was no pre-incubation time of the enzyme with inhibitor solution. The activity of the enzyme was determined by monitoring the increase in the absorption caused by the oxidation of linoleic acid at 234 nm and 25°C (ε = 25 000 M^−1 cm^−1). One standard solution of complexes in DMSO (10^-2 M) was used for theinhibition activity experiments (three sets). In this case, the substrate concentration was kept constant (0.3 mM), while the amounts of buffer and inhibitor solutions varied according to the inhibitor final concentration needed(0.5-30 μM or 0.15-9 μL from standard solutions). The total experiment volume was 3 cm^3.
Affinity data for this assay
 

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