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Assay Method Information

Assay Name:  Enzymatic Assay
Description:  Enzymatic activity is measured by contacting the enzyme with a suitable substrate, such as fructose 1,6-bisphosphate, and measuring formation of a product such as dihydroxyacetone phosphate (DHAP) or glyceraldehyde 3-phosphate (GAP). If the activity is measurably lower in the presence of the candidate compound than in its absence, then the compound has FBA inhibitory activity and is a candidate antibiotic and antiparasitic compound. The compound may also be screened with recombinant or isolated Class I FBA (for example, of human or mammalian origin) to determine cross-inhibition.
Affinity data for this assay
 

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Last update November 1, 2007
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