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Assay Method Information

Assay Name:  PTP1B Activity Assay
Description:  PTP1B was diluted before the experiment to 1.2 μg/mL in Tris buffer, pH7.6 (10 mM Tris, 1.0 mM EDTA, 3.0 mM DTT, 0.01% w/v NaN3). The tested compounds were dissolved in DMSO. The enzyme reaction buffer was Tris buffer, pH 7.5 (50 mM Tris, 0.15 M NaCl, 3 mM DTT). A typical 10 μL of tested compounds was added to the enzyme solution (50 μL), and the resulting mixture was preincubated at 37C for 30 min. The enzyme reaction was initiated by the addition of 8 mM p-NPP (40 μL). The concentration of p-nitrophenol from the reaction mixtures was recorded at 405 nm, 15-min intervals for 120 min. SOV was used as a positive control in this study.
Affinity data for this assay
 

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Last update November 1, 2007
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