| Assay Method Information | |
| | In Vitro Assay |
| Description: | Small molecule inhibitors were obtained from ChemDiv and resuspended in DMSO. Compound TDLR-505 was independently synthesized and structure confirmed by mass spectrometry analysis. Human RPA was purified using an E. coli over expression system as previously described (7). EMSAs were performed in 20 μL reactions containing 25 nM RPA, 25 nM 5′[32P]-labeled 34-base pair DNA as previously described (7). The final concentration of DMSO was 1%. |
| Affinity data for this assay | |
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