Assay Method Information

Assay Name:  Cell-based Screening of Chemical Libraries for Characterization of Necroptosis Inhibitors
Description:  TNF-α can induce necroptosis in Jurkat cells (human T lymphocytes) when FADD was deleted. This model was used to screen various libraries of chemical compounds for characterization of new inhibitors of cellular necroptosis. Details on this cell-based assay can be found in Miao and Degterev (Methods Mol. Biol. 2009, 559, 79-93). The Jurkat FADD-deficient I 2.1 cell line used was purchased from ATCC and was maintained in RPMI 1640 medium (Gibco) containing Glutamax and 15% fetal calf serum (Life Technology). Necroptosis was induced by addition of 10 ng/ml of human recombinant TNF-α (Life Technology). Necrostatin-1 (Nec-1, Enzo Life Sciences) was used as model necroptosis inhibitor. Cells were maintained in 75 cm2 flask and passed every 2 or 3 days. Chemical collections analysed were formatted in 96-well plates with 80 molecules per plates at 10 mM in 100% DMSO. For each collection plate, 2 cell plates were seeded (one necroptosis-induced with TNF-α and the other without TNF-α). Cells were seeded at 20000 cells/well, in 40 μl of medium, in a 96-well clear, flat bottom plate (CytoOne, Starlab) before treatment. Then, 40 μl of medium with or without TNF-α at 25 ng/ml were added to all wells in the corresponding plate. Immediately after TNF-α addition, 20 μl of diluted compound at 50 μM were added to the plates. Final concentration of each chemical compound was 10 μM at 0.1% DMSO. Four positives (Nec-1 at 10 μM final) and four negative (DMSO) controls were used in each plate to validate the assay. Cells were incubated at 37° C., 5% CO2 for 24 hours before performing MTS viability assay, described hereafter. Compounds were diluted before to treat cells. Liquid handling was performed using the Nimbus Microlab liquid handler (Hamilton Robotics) under microbiological safety workbench. The 10 mM compounds were diluted at 50 μM directly in cell medium.
Affinity data for this assay
 

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