Assay Method Information | |
| Radiometric Filter Plate MLK3 Assay |
Description: | 200 ng (130 nM) MLK3 (Dundee, DU8313) was incubated with 1 μM inactive MKK7b (Dundee, DU703) in the presence of 2 μM cold ATP (Km) and 0.5 μCi/assay 33P ATP and appropriate concentrations of compounds. After a twenty minute incubation, the reactions were washed through filter plates and read on a scintillation counter. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |