| Assay Method Information | |
| | Antiviral activity assays |
| Description: | Briefly, Vero E6 cells (ATCC CRL-1586) seeded at 3.5 × 104 cells/well in 96-well plates were inoculated with 50 μ l of serial tenfold dilutions of cell culture supernatant from treated cells. The inoculum was removed after 1 h and replaced by a 1.5% methylcellulose-DMEM-5% FBS overlay. Following incubation for 24 h, cells were 445 inactivated and fixed with 4.5% formaldehyde. Infected cells were detected using an antibody against SARS-CoV-2 NP (ThermoFischer, PA5-81794). Foci were counted using an AID ELISpot reader from Mabtech. The cytotoxic concentrations that reduced cell growth by 50% (CC50) and the effective concentrations that reduced infectious particles or vRNA by 50% (EC50) were calculated by fitting the data to the sigmoidal function using GraphPad Prism 450 version 8.00 (GraphPad Software, La Jolla California USA, www.graphpad.com). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |