| Assay Method Information | |
| | hCA IX Enzyme Inhibition Assay |
| Description: | An Applied Photophysics stopped-flow instrument has been used for assaying the CA-catalyzed CO2 hydration activity. Phenol red has been used as indicator, working at the absorbance maximum of 557 nm. The inhibition constants were obtained by nonlinear least-squares methods. The IC50 was obtained by using curve-fitting algorithm. |
| Affinity data for this assay | |
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