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Assay Method Information

Assay Name:  Biological Assay
Description:  Two types of enzyme assays were developed to follow TgCDPK1 activity, a radiometric scintillation proximity assay measured the labeled γ-phosphate of ATP added to a biotinylated peptide substrate and an ATP consumption assay where ATP consumption was monitored by luciferase and light production (KinaseGloŽ, Promega Corp., Madison, Wis.). Both assays gave similar results for calcium dependence, Km of substrates (less than 2-fold differences in Km values; see Table 6 FIGS. 6, 7, 8, and 9), and inhibitor concentrations for 50% enzyme inhibition.
Affinity data for this assay
 

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Last update November 1, 2007
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