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Assay Method Information

Assay Name:  Jak2 Inhibition Assay
Description:  Compounds of Formula I were screened for their ability to inhibit Jak2 using the general enzyme inhibition assay method, in which the assay mixture contained 50 μM ATP, 10 μM OmniaŽ Y7 peptide (Catalog #IVGN KNZ3071C, Invitrogen Corporation, Carlsbad, Calif.) and 4 nM Jak2 in a total volume of 20 μL. Human Jak2 kinase domain comprising amino acids 808-1132 was purchased from Invitrogen Corporation, Carlsbad, Calif. (catalog #IVGN PV4210).
Affinity data for this assay
 

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Last update November 1, 2007
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